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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular <t>fluorescence</t> intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).
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a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular fluorescence intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).

Journal: bioRxiv

Article Title: Optimization and Parallelization of Sorting by Interfacial Tension (SIFT) for High-Throughput Metabolic Cell Sorting

doi: 10.64898/2026.03.11.710714

Figure Lengend Snippet: a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular fluorescence intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).

Article Snippet: On-chip images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) equipped with a 4X objective, a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ).

Techniques: Fluorescence